Development of a real-time PCR assay for detection of gyrA mutations associated with reduced susceptibility to ciprofloxacin in Salmonella enterica serovar typhi and paratyphi A

Masatomo Morita; Kenitiro Ito; Kenji Hirose; Hideyuki Takahashi; Ken Shimuta; Jun Terajima; Makoto Ohnishi; Makoto Harada; Mitsuhiro Matsuzaki; Haruo Watanabe; Hidemasa Izumiya

doi:10.1111/j.1348-0421.2006.tb03843.x

Development of a real-time PCR assay for detection of gyrA mutations associated with reduced susceptibility to ciprofloxacin in Salmonella enterica serovar typhi and paratyphi A

Microbiol Immunol. 2006;50(9):707-11. doi: 10.1111/j.1348-0421.2006.tb03843.x.

Authors

Masatomo Morita¹, Kenitiro Ito, Kenji Hirose, Hideyuki Takahashi, Ken Shimuta, Jun Terajima, Makoto Ohnishi, Makoto Harada, Mitsuhiro Matsuzaki, Haruo Watanabe, Hidemasa Izumiya

Affiliation

¹ Department of Bacteriology, National Institute of Infectious Diseases, Tokyo, Japan.

PMID: 16985292
DOI: 10.1111/j.1348-0421.2006.tb03843.x

Abstract

A real-time PCR assay with the cycling probe method was used to detect mutations at codons 83 and 87 in the DNA gyrase A subunit encoded by gyrA in Salmonella enterica serovar Typhi and Paratyphi A clinical isolates. The susceptibility estimated from the results of the gyrA mutation assay was consistent with that identified by the culture method using an E-test. This assay allows rapid screening of S. enterica serovar Typhi and Paratyphi A with reduced susceptibility to ciprofloxacin.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Anti-Bacterial Agents / pharmacology*
Ciprofloxacin / pharmacology*
DNA Gyrase / genetics*
Drug Resistance, Bacterial
Humans
Mutation
Paratyphoid Fever / microbiology
Polymerase Chain Reaction / methods*
Salmonella paratyphi A / drug effects*
Salmonella paratyphi A / genetics
Salmonella typhi / drug effects*
Salmonella typhi / genetics
Typhoid Fever / microbiology

Substances

Anti-Bacterial Agents
Ciprofloxacin
DNA Gyrase