Endoluminal closure of large perforations of colon with clips in a porcine model (with video)

Gastrointest Endosc. 2006 Oct;64(4):640-6. doi: 10.1016/j.gie.2006.02.040. Epub 2006 Jun 12.

Abstract

Background: Endoluminal clip closure of small perforations of the colon is possible. It is unclear whether large perforations of the colon can be closed with clips deployed through a colonoscope.

Objectives: To evaluate the technical feasibility and outcome of endoluminal closure of large perforations of the colon with clips.

Design: Pilot study.

Subjects: Eight pigs.

Interventions: Endoluminal clip closure of large perforations of the colon.

Main outcome measurements: Technical feasibility of endoluminal closure was evaluated in 8 animals. A dye leak test was performed to evaluate quality of endoluminal sealing immediately after closure and 2 weeks after closure. The animals in the survival group were monitored daily for 2 weeks for sepsis and peritonitis. Necropsy was done on day 14 to check for fecal peritonitis, wound dehiscence, and histological healing of perforation.

Results: Endoscopic closure of colon perforation was successful in 6 of 8 animals; in 2 animals closure was unsuccessful due to prolapse of adjacent viscera into the colon (n = 1) and severe bleeding that obscured the view (n = 1). There was a leak-proof sealing of the perforation site in 2 animals tested immediately and in all the animals tested (n = 3) 2 weeks after closure. All 4 animals in the survival group recovered well, without any sepsis or peritonitis. Necropsy did not reveal fecal peritonitis or pericolonic abscess formation. The perforation site demonstrated a thin scar and there was histological healing.

Conclusions: Endoluminal application of clips is successful in the closure of a large perforation of the colon in a porcine model.

MeSH terms

  • Animals
  • Colonic Diseases / pathology
  • Colonic Diseases / surgery*
  • Colonoscopy*
  • Disease Models, Animal*
  • Feasibility Studies
  • Intestinal Mucosa / pathology
  • Intestinal Mucosa / surgery
  • Intestinal Perforation / pathology
  • Intestinal Perforation / surgery*
  • Peritoneum / pathology
  • Peritonitis / pathology
  • Postoperative Complications / pathology
  • Surgical Instruments*
  • Swine
  • Wound Healing / physiology