Confirmation of sex-specific transcripts from Ancylostoma caninum adult worms by semi-quantitative RT-PCR

R R Miranda; L Clara e Silva; H A Santos; E M Rabelo

doi:10.1016/j.rvsc.2006.07.002

Confirmation of sex-specific transcripts from Ancylostoma caninum adult worms by semi-quantitative RT-PCR

Res Vet Sci. 2007 Apr;82(2):215-7. doi: 10.1016/j.rvsc.2006.07.002. Epub 2006 Sep 26.

Authors

R R Miranda¹, L Clara e Silva, H A Santos, E M Rabelo

Affiliation

¹ Departamento de Parasitologia, Instituto de Ciências Biológicas, Universidade Federal de Minas Gerais, Minas Gerais, Brazil.

PMID: 17005221
DOI: 10.1016/j.rvsc.2006.07.002

Abstract

In a previous work Ancylostoma caninum sex-specific genes had being identified, however without confirmation, by using an non specific RT-PCR comparing male and female cDNA. In this work more fragments had been identified and a semi-quantitative RT-PCR carried out in order to confirm the sex-specific character of the transcripts obtained. Four fragments were confirmed as sex-specific or as being expressed more in one sex than the other.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Ancylostoma / genetics*
Ancylostoma / isolation & purification
Ancylostomiasis / parasitology
Ancylostomiasis / veterinary*
Animals
Dog Diseases / parasitology*
Dogs
Female
Male
RNA, Helminth / chemistry
RNA, Helminth / genetics
Reverse Transcriptase Polymerase Chain Reaction / veterinary
Sequence Analysis, Protein
Sex Characteristics

Substances

RNA, Helminth