Abstract
A putative core protein derived from hepatitis C virus was expressed in E. coli. More than 5% of the total protein expressed in the bacteria after induction by isopropylthio-beta-D-galactoside was shown to be the expected protein. Western blotting with this E. coli lysate proved to be more efficient than ELISA with a non-structural viral protein, C100, to detect infection of hepatitis C virus in the sera of patients with non-A, non-B chronic hepatitis, hepatocellular carcinoma as well as in sera from healthy persons.
Publication types
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Research Support, Non-U.S. Gov't
MeSH terms
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Base Sequence
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Blotting, Western
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Cloning, Molecular / methods
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Escherichia coli / genetics*
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Genes, Viral
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Hepacivirus / genetics
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Hepacivirus / isolation & purification*
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Hepatitis C / diagnosis*
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Humans
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Molecular Sequence Data
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Oligonucleotide Probes
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Plasmids
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RNA, Viral / genetics
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RNA, Viral / isolation & purification
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RNA-Directed DNA Polymerase / metabolism
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Viral Structural Proteins / analysis
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Viral Structural Proteins / genetics*
Substances
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Oligonucleotide Probes
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RNA, Viral
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Viral Structural Proteins
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RNA-Directed DNA Polymerase