Rapid simultaneous detection by real-time PCR of cytomegalovirus UL97 mutations in codons 460 and 520 conferring ganciclovir resistance

Katharina Göhring; Elfriede Mikeler; Gerhard Jahn; Klaus Hamprecht

doi:10.1128/JCM.01141-06

Rapid simultaneous detection by real-time PCR of cytomegalovirus UL97 mutations in codons 460 and 520 conferring ganciclovir resistance

J Clin Microbiol. 2006 Dec;44(12):4541-4. doi: 10.1128/JCM.01141-06. Epub 2006 Oct 11.

Authors

Katharina Göhring¹, Elfriede Mikeler, Gerhard Jahn, Klaus Hamprecht

Affiliation

¹ Institute of Medical Virology and Epidemiology of Viral Diseases, University Hospital of Tübingen, Elfriede-Aulhorn-Str. 6, D-72076 Tübingen, Germany.

Abstract

Ganciclovir (GCV) resistance is an emerging problem for transplant recipients. A sensitive and rapid real-time PCR approach for simultaneous and semiquantitative detection of human cytomegalovirus (HCMV) UL97 mutations in codons 460/520 was established by LightCycler and confirmed by restriction fragment length polymorphism and sequencing. Results from HCMV laboratory strains were compared with results from 11 GCV-resistant clinical isolates.

MeSH terms

Antiviral Agents / pharmacology*
Codon / genetics
Cytomegalovirus / drug effects
Cytomegalovirus / genetics*
DNA Fingerprinting
DNA, Viral / genetics
Drug Resistance, Viral / genetics*
Ganciclovir / pharmacology*
Mutation*
Phosphotransferases (Alcohol Group Acceptor) / genetics*
Polymerase Chain Reaction / methods*
Polymorphism, Restriction Fragment Length
Sequence Analysis, DNA

Substances

Antiviral Agents
Codon
DNA, Viral
Phosphotransferases (Alcohol Group Acceptor)
ganciclovir kinase
Ganciclovir