Feasibility of freeze-dried sera for serological and molecular biological detection of hepatitis B and C viruses

J Clin Microbiol. 2006 Dec;44(12):4593-5. doi: 10.1128/JCM.00621-06. Epub 2006 Oct 11.

Abstract

We compared hepatitis B virus (HBV) surface antigen, anti-hepatitis C virus (HCV) antibody, and HCV RNA quantification in frozen and freeze-dried serum samples to assess the usefulness of freeze-dried sera for detection of HBV and HCV. The results indicated that freeze-dried sera as well as frozen sera can be useful for serological and molecular biological analyses of HBV and HCV.

Publication types

  • Comparative Study
  • Research Support, Non-U.S. Gov't
  • Research Support, U.S. Gov't, Non-P.H.S.

MeSH terms

  • Freeze Drying
  • Hepacivirus / genetics
  • Hepacivirus / immunology
  • Hepacivirus / isolation & purification*
  • Hepatitis B / diagnosis
  • Hepatitis B / virology
  • Hepatitis B Surface Antigens / analysis
  • Hepatitis B virus / genetics
  • Hepatitis B virus / immunology
  • Hepatitis B virus / isolation & purification*
  • Hepatitis C / diagnosis
  • Hepatitis C / virology
  • Hepatitis C Antibodies / analysis
  • Humans
  • RNA, Viral / analysis
  • Reproducibility of Results
  • Sensitivity and Specificity
  • Serum / virology*
  • Specimen Handling / methods*

Substances

  • Hepatitis B Surface Antigens
  • Hepatitis C Antibodies
  • RNA, Viral