Abstract
We present a PCR method targeting the 23S-5S internal transcribed spacer coupled with reverse line blotting that allows Rickettsia species detection and identification in a single step. The method is highly sensitive and specific in identifying Rickettsia species from both patient and environmental samples. The generic approach used allowed us to identify new pathogens.
Publication types
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Evaluation Study
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Research Support, Non-U.S. Gov't
MeSH terms
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Animals
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Bacteriological Techniques*
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DNA, Bacterial / genetics
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DNA, Ribosomal Spacer / genetics
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Humans
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Molecular Sequence Data
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Nucleic Acid Hybridization
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Phylogeny
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Polymerase Chain Reaction / methods*
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Rickettsia / isolation & purification*
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Rickettsia Infections / diagnosis
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Rickettsia Infections / microbiology*
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Sensitivity and Specificity
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Sequence Analysis, DNA
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Sequence Homology, Nucleic Acid
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Siphonaptera / microbiology
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Ticks / microbiology
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Water Microbiology
Substances
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DNA, Bacterial
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DNA, Ribosomal Spacer