Abstract
A high rate of double point mutations in gyrA (56% of 87 ofloxacin-resistant Mycobacterium tuberculosis clinical isolates) indicates the emergence of fluoroquinolone resistance. This is the first report to describe denaturing high-pressure liquid chromatography analysis of mutations in gyrA of M. tuberculosis in a large number of clinical isolates.
Publication types
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Research Support, Non-U.S. Gov't
MeSH terms
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Anti-Bacterial Agents / pharmacology*
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China
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Chromatography, High Pressure Liquid
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DNA Gyrase / genetics*
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DNA Mutational Analysis
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DNA, Bacterial / genetics
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Drug Resistance, Bacterial / genetics*
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Humans
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Mutation, Missense
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Mycobacterium tuberculosis / drug effects*
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Mycobacterium tuberculosis / genetics
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Mycobacterium tuberculosis / isolation & purification
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Ofloxacin / pharmacology*
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Point Mutation
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Sequence Analysis, DNA
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Tuberculosis / microbiology*
Substances
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Anti-Bacterial Agents
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DNA, Bacterial
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Ofloxacin
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DNA Gyrase