[Genetic environment of CTX-M-2 in Klebsiella pneumoniae isolates from hospitalized patients in Uruguay]

Rev Argent Microbiol. 2006 Apr-Jun;38(2):84-8.
[Article in Spanish]

Abstract

We studied two CTX-M-2-producing Klebsiella pneumoniae clinical strains, K96005 and K13, isolated from hospitalized patients in Uruguay, during 1996 and 2003, respectively. The genomic surroundings of bla(CTX-M-2) were characterized by PCR-mapping and DNA sequencing. Our results show that blaCTX-M-2 is included in a complex class-1 integron (InK13), associated with an orf513 in both isolates. The genetic array of the integron, aac(6')-lb, bla(OxA,2), orfD (gene cassette region), associated with an orf513-bla(CTX-M-2), seems to be widely disseminated over the Rio de la Plata region.

MeSH terms

  • Base Sequence
  • DNA, Bacterial / analysis*
  • Drug Resistance, Bacterial / genetics*
  • Humans
  • Integrons
  • Klebsiella Infections / microbiology
  • Klebsiella pneumoniae / enzymology*
  • Molecular Sequence Data
  • Polymerase Chain Reaction
  • Uruguay
  • beta-Lactamases / genetics*

Substances

  • DNA, Bacterial
  • beta-lactamase CTX-2
  • beta-lactamase CTX-M-2, Klebsiella pneumoniae
  • beta-Lactamases

Associated data

  • GENBANK/AM040449
  • GENBANK/AM040450