Abstract
We studied the molecular epidemiology of dengue virus type 3 (DENV-3) in Brazil and Paraguay by analyzing the 5' and 3' untranslated regions (5' and 3'UTRs) and the E protein gene of viruses isolated between 2002 and 2004. Both 5' and 3'UTRs were highly conserved. However, the 3'UTR of two isolates from Brazil contained eight nucleotide deletions compared with the remaining 26 viruses. Phylogenetic analyses suggested that DENV-3 was introduced into Brazil from the Caribbean Islands at least twice and into Paraguay from Brazil at least three times.
Publication types
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Research Support, Non-U.S. Gov't
MeSH terms
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3' Untranslated Regions / chemistry
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Amino Acid Sequence
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Base Sequence
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Brazil / epidemiology
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Consensus Sequence
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DNA, Complementary / chemistry
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DNA, Viral / chemistry
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Dengue / epidemiology*
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Dengue / virology
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Dengue Virus / classification
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Dengue Virus / genetics*
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Humans
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Paraguay / epidemiology
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Phylogeny
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RNA, Viral / genetics
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RNA, Viral / isolation & purification
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Reverse Transcriptase Polymerase Chain Reaction
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Viral Envelope Proteins / chemistry
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Viral Envelope Proteins / genetics*
Substances
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3' Untranslated Regions
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DNA, Complementary
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DNA, Viral
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E protein, Dengue virus type 3
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RNA, Viral
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Viral Envelope Proteins