Use of loop electrosurgical excision procedure specimens to provide tissue fractions for immunologic analyses

J Low Genit Tract Dis. 2003 Oct;7(4):285-9. doi: 10.1097/00128360-200310000-00010.

Abstract

Objective: To determine if dividing loop electrosurgical excision procedure (LEEP) specimens to provide tissue for research increases rates of LEEP specimen misdiagnosis and recurrent cervical intraepithelial neoplasia.

Materials and methods: In this chart review, 42 women with biopsy-confirmed cervical intraepithelial neoplasia (CIN) 2,3 had up to 20% of their LEEP specimens sectioned and used for immunologic analysis. The remainder of each specimen was assessed routinely. Follow-up cytologic analyses and cervical biopsies also were assessed. This cohort was compared with a control cohort of 80 patients with biopsy confirmed CIN 2,3 whose LEEP specimens were not divided. Statistical significance was defined as a p value of < .05.

Results: There were no statistically significant differences between the groups with regard to histologic assessment of LEEP specimens or follow-up outcomes.

Conclusions: Use of up to 20% of LEEP specimens for research purposes neither adversely affects histologic evaluation of LEEP specimens nor leads to poorer follow-up outcomes.