Abstract
In New York State during winter 2004, there was a high incidence of influenza-like illness that tested negative both for influenza virus, by molecular methods, and for other respiratory viruses, by virus culture. Concern that a novel pathogen might be implicated led us to implement a new multiplex diagnostic tool. MassTag polymerase chain reaction resolved 26 of 79 previously negative samples, revealing the presence of rhinoviruses in a large proportion of samples, half of which belonged to a previously uncharacterized genetic clade. In some instances, knowledge of the detected viral and/or bacterial (co)infection could have altered clinical management.
Publication types
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Research Support, N.I.H., Extramural
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Research Support, Non-U.S. Gov't
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Research Support, U.S. Gov't, P.H.S.
MeSH terms
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Adolescent
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Adult
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Aged
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Aged, 80 and over
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Bacteria / classification
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Bacteria / genetics
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Bacteria / isolation & purification*
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Child
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Child, Preschool
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Humans
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Infant
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Middle Aged
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Molecular Epidemiology
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Molecular Sequence Data
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New York / epidemiology
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Phylogeny
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Picornaviridae Infections / epidemiology
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Picornaviridae Infections / virology
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Polymerase Chain Reaction / methods*
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Respiratory Tract Infections / epidemiology
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Respiratory Tract Infections / microbiology*
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Respiratory Tract Infections / virology*
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Rhinovirus / classification
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Rhinovirus / genetics
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Rhinovirus / isolation & purification*
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Sequence Analysis, DNA
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Viral Proteins / genetics
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Viruses / classification
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Viruses / genetics
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Viruses / isolation & purification*
Associated data
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GENBANK/DQ875920
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GENBANK/DQ875921
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GENBANK/DQ875922
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GENBANK/DQ875923
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GENBANK/DQ875924
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GENBANK/DQ875925
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GENBANK/DQ875926
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GENBANK/DQ875927
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GENBANK/DQ875928
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GENBANK/DQ875929
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GENBANK/DQ875930
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GENBANK/DQ875931
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GENBANK/DQ875932