Hepatocyte growth factor-induced Ras activation requires ERM proteins linked to both CD44v6 and F-actin

Mol Biol Cell. 2007 Jan;18(1):76-83. doi: 10.1091/mbc.e06-08-0674. Epub 2006 Oct 25.

Abstract

In several types of cells, the activation of the receptor tyrosine kinase c-Met by its ligand hepatocyte growth factor (HGF) requires the coreceptor CD44v6. The CD44 extracellular domain is necessary for c-Met autophosphorylation, whereas the intracellular domain is required for signal transduction. We have already shown that the CD44 cytoplasmic tail recruits ezrin, radixin and moesin (ERM) proteins to the complex of CD44v6, c-Met, and HGF. We have now defined the function of the ERM proteins and the step they promote in the signaling cascade. The association of ERM proteins to the coreceptor is absolutely required to mediate the HGF-dependent activation of Ras by the guanine nucleotide exchange factor Sos. The ERM proteins need, in addition, to be linked to the actin cytoskeleton to catalyze the activation of Ras. Thus, we describe here a new function of the cytoskeleton. It is part of a "signalosome" complex that organizes the activation of Ras by Sos. So far the cytoskeleton has mainly been identified as a "responder" to signal transduction. Here, we show now that F-actin acts as an "inducer" that actively organizes the signaling cascade.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Actins / metabolism*
  • Animals
  • Cytoskeletal Proteins / metabolism*
  • Cytoskeleton / drug effects
  • Glycoproteins / metabolism*
  • HT29 Cells
  • Hepatocyte Growth Factor / pharmacology*
  • Humans
  • Hyaluronan Receptors / metabolism*
  • Membrane Proteins / metabolism
  • Microfilament Proteins / metabolism
  • Protein Binding / drug effects
  • Protein Structure, Tertiary / drug effects
  • Protein Transport / drug effects
  • Proto-Oncogene Proteins c-met / metabolism
  • Proto-Oncogene Proteins p21(ras) / metabolism*
  • Rats
  • Signal Transduction / drug effects
  • Son of Sevenless Proteins / metabolism

Substances

  • Actins
  • CD44v6 antigen
  • Cytoskeletal Proteins
  • Glycoproteins
  • Hyaluronan Receptors
  • Membrane Proteins
  • Microfilament Proteins
  • Son of Sevenless Proteins
  • ezrin
  • moesin
  • radixin
  • Hepatocyte Growth Factor
  • Proto-Oncogene Proteins c-met
  • Proto-Oncogene Proteins p21(ras)