Abstract
Our whole-genome microarray studies of Neisseria meningitidis MC58 previously identified a set of 153 genes whose transcription was activated during growth in iron. In this study, Fur-mediated regulation of the iron-activated nspA gene was confirmed, whereas iron-activated regulation of the secY gene was demonstrated to be Fur independent. Analysis of the Fur binding sequences in the nspA gene and an additional iron-activated and Fur-regulated gene identified a hexameric (G/T)ATAAT unit in the operator regions of these genes similar to that observed in Fur- and iron-repressed genes. These studies indicate that the expression of the iron-activated nspA and secY genes in N. meningitidis occur by Fur-dependent and -independent mechanisms, respectively.
Publication types
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Research Support, N.I.H., Extramural
MeSH terms
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Bacterial Outer Membrane Proteins / genetics*
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Bacterial Outer Membrane Proteins / metabolism
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Bacterial Proteins / genetics*
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Bacterial Proteins / metabolism
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Base Sequence
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DNA Footprinting
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Electrophoretic Mobility Shift Assay
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Gene Expression Profiling
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Gene Expression Regulation, Bacterial*
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Genes, Bacterial / genetics
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Iron / metabolism
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Neisseria meningitidis, Serogroup B / genetics*
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Neisseria meningitidis, Serogroup B / metabolism
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Oligonucleotide Array Sequence Analysis
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Oligonucleotides / genetics
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Oligonucleotides / metabolism
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Protein Binding
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Repressor Proteins / genetics*
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Repressor Proteins / metabolism
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Reverse Transcriptase Polymerase Chain Reaction
Substances
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Bacterial Outer Membrane Proteins
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Bacterial Proteins
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NspA protein, Neisseria
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Oligonucleotides
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Repressor Proteins
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ferric uptake regulating proteins, bacterial
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Iron