Hepatitis C virus (HCV) NS5B nonnucleoside inhibitors specifically block single-stranded viral RNA synthesis catalyzed by HCV replication complexes in vitro

Antimicrob Agents Chemother. 2007 Jan;51(1):338-42. doi: 10.1128/AAC.00498-06. Epub 2006 Nov 6.

Abstract

Replication complexes of hepatitis C virus synthesized two major species of viral RNA in vitro, double stranded and single stranded. NS5B nonnucleoside inhibitors inhibited dose dependently the synthesis of single-stranded RNA but not double-stranded RNA. Moreover, replication complexes carrying a mutation resistant to a nonnucleoside inhibitor lost their susceptibilities to the inhibitor.

MeSH terms

  • Benzimidazoles / chemistry
  • Benzimidazoles / pharmacology
  • Benzothiadiazines / chemistry
  • Benzothiadiazines / pharmacology
  • Dose-Response Relationship, Drug
  • Hepacivirus / drug effects*
  • Hepacivirus / genetics*
  • Molecular Structure
  • Mutation
  • RNA, Viral / genetics*
  • RNA, Viral / metabolism
  • Templates, Genetic
  • Viral Nonstructural Proteins / antagonists & inhibitors*
  • Viral Nonstructural Proteins / genetics
  • Viral Nonstructural Proteins / metabolism
  • Virus Replication / drug effects

Substances

  • Benzimidazoles
  • Benzothiadiazines
  • RNA, Viral
  • Viral Nonstructural Proteins
  • benzimidazole
  • NS-5 protein, hepatitis C virus