Alpha-aminothiazole-gamma-aminobutanoic amides as potent, small molecule CCR2 receptor antagonists

Bioorg Med Chem Lett. 2007 Jan 15;17(2):309-14. doi: 10.1016/j.bmcl.2006.10.059. Epub 2006 Oct 25.

Abstract

A series of racemic and homochiral alpha-aminothiazole-gamma-aminobutyroamides that display high affinities for human and murine CCR2 and functional antagonism by inhibition of monocyte recruitment are described. A representative example is (2S)-2-[2-(acetylamino)-1,3-thiazol-4-yl]-N-[3-methyl-5-(trifluoromethyl)benzyl]-4-(4-phenylpiperidin-1-yl)butanamide, which shows 5 nM affinity for human monocytes and CHO cells expressing the human CCR2b receptor. It also inhibited MCP-1 initiated chemotaxis of human monocytes with an IC50 of 0.69 nM.

MeSH terms

  • Animals
  • CHO Cells
  • Chemokine CCL2 / antagonists & inhibitors
  • Chemokine CCL2 / metabolism
  • Chemotaxis / drug effects
  • Chromatography, High Pressure Liquid
  • Cricetinae
  • Cricetulus
  • Dogs
  • Drug Design
  • Humans
  • In Vitro Techniques
  • Indicators and Reagents
  • Membranes / metabolism
  • Monocytes / drug effects
  • Monocytes / metabolism
  • Neurokinin-1 Receptor Antagonists
  • Rats
  • Rats, Sprague-Dawley
  • Receptors, CCR2
  • Receptors, Chemokine / antagonists & inhibitors*
  • Receptors, Chemokine / drug effects
  • Stereoisomerism
  • Structure-Activity Relationship

Substances

  • CCL2 protein, human
  • CCR2 protein, human
  • Ccr2 protein, rat
  • Chemokine CCL2
  • Indicators and Reagents
  • Neurokinin-1 Receptor Antagonists
  • Receptors, CCR2
  • Receptors, Chemokine