Cloning and sequence analysis of a cDNA encoding human non-selective type of endothelin receptor

M Nakamuta; R Takayanagi; Y Sakai; S Sakamoto; H Hagiwara; T Mizuno; Y Saito; S Hirose; M Yamamoto; H Nawata

doi:10.1016/0006-291x(91)91944-8

Cloning and sequence analysis of a cDNA encoding human non-selective type of endothelin receptor

Biochem Biophys Res Commun. 1991 May 31;177(1):34-9. doi: 10.1016/0006-291x(91)91944-8.

Authors

M Nakamuta¹, R Takayanagi, Y Sakai, S Sakamoto, H Hagiwara, T Mizuno, Y Saito, S Hirose, M Yamamoto, H Nawata

Affiliation

¹ Third Department of Internal Medicine, Faculty of Medicine, Kyushu University, Japan.

PMID: 1710450
DOI: 10.1016/0006-291x(91)91944-8

Abstract

A cDNA encoding non-selective type (ETB) of endothelin receptor was isolated from a human liver cDNA library. The cDNA had an open reading frame encoding a protein of 442 amino acid residues with a relative Mr of 49,643. The deduced amino acid sequence of human ETB receptor was 88% and 64% identical to those of rat lung ETB receptor and bovine lung ET-1-specific (ETA) receptor, respectively, and contained a relatively long and proline-rich extracellular N-terminal region in addition to a significant similarity with the G protein-coupled receptor super-family with seven transmembrane segments.

Publication types

Comparative Study
Research Support, Non-U.S. Gov't

MeSH terms

Adult
Amino Acid Sequence
Animals
Base Sequence
Blotting, Northern
Cloning, Molecular
DNA / genetics*
DNA / isolation & purification
Endothelins / metabolism*
Humans
Liver / metabolism
Molecular Sequence Data
Oligonucleotide Probes
Peptide Fragments
Poly A / genetics
Poly A / isolation & purification
RNA / genetics
RNA / isolation & purification
RNA, Messenger
Rats
Receptors, Cell Surface / genetics*
Receptors, Endothelin
Sequence Homology, Nucleic Acid

Substances

Endothelins
Oligonucleotide Probes
Peptide Fragments
RNA, Messenger
Receptors, Cell Surface
Receptors, Endothelin
Poly A
RNA
DNA