Abstract
In legumes, Nod-factor signaling by rhizobia initiates the development of the nitrogen-fixing nodule symbiosis, but the direct cell division stimulus that brings about nodule primordia inception in the root cortex remains obscure. We showed that Lotus japonicus plants homozygous for a mutation in the HYPERINFECTED 1 (HIT1) locus exhibit abundant infection-thread formation but fail to initiate timely cortical cell divisions in response to rhizobial signaling. We demonstrated that the corresponding gene encodes a cytokinin receptor that is required for the activation of the nodule inception regulator Nin and nodule organogenesis.
Publication types
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Research Support, Non-U.S. Gov't
MeSH terms
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Alleles
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Alphaproteobacteria / growth & development
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Alphaproteobacteria / physiology*
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Cell Division
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Cytokinins / metabolism*
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Cytokinins / pharmacology
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Genes, Plant
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Histidine Kinase
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Lipopolysaccharides / metabolism
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Lotus / genetics
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Lotus / microbiology*
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Lotus / physiology*
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Mutation
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Nitrogen Fixation
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Plant Epidermis / cytology
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Plant Epidermis / metabolism
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Plant Proteins / genetics
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Plant Proteins / metabolism
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Plant Roots / cytology
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Plant Roots / metabolism
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Plant Roots / microbiology
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Protein Kinases / genetics*
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Protein Kinases / metabolism*
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Receptors, Cell Surface / genetics
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Receptors, Cell Surface / metabolism
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Root Nodules, Plant / cytology
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Root Nodules, Plant / growth & development*
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Root Nodules, Plant / microbiology
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Signal Transduction*
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Symbiosis
Substances
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Cytokinins
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Lipopolysaccharides
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NIN protein, Lotus japonicus
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Plant Proteins
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Receptors, Cell Surface
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Protein Kinases
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Histidine Kinase