Imaging using parallel integrals in optical projection tomography

Phys Med Biol. 2006 Dec 7;51(23):6023-32. doi: 10.1088/0031-9155/51/23/005. Epub 2006 Oct 30.

Abstract

We develop and demonstrate improved image-forming optics for optical projection tomography (OPT), with which the parallel integral throughout an object can be obtained. This method results in an improved resolution for OPT images, especially for the cross sections far from the optical axis of the image-forming optics. We find the optimal configuration used in our OPT system by use of a point spread function and simulation technique. The new method is validated by both numerical simulations and experimental results. The spatial resolution of the OPT system presented is approximately 40 microm.

MeSH terms

  • Animals
  • Biophysical Phenomena
  • Biophysics
  • Chick Embryo
  • Diagnostic Imaging / instrumentation
  • Diagnostic Imaging / statistics & numerical data
  • Humans
  • Models, Statistical
  • Phantoms, Imaging
  • Tomography, Optical / instrumentation*
  • Tomography, Optical / statistics & numerical data