Abstract
The development of ECL-Plex CyDye-conjugated secondary antibodies allows the advancement of conventional Western blotting, opening up possibilities for highly sensitive and quantitative protein confirmation and identification. We report a novel proteomic method to simultaneously visualise the total protein profile as well as the specific immunodetection of an individual protein species by combining cyanine CyDye pre-labelled proteins and antibody immunoblotting. This technique proposes to revolutionise both 2-D immunoprobing and protein confirmation following MS analysis.
Publication types
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Research Support, Non-U.S. Gov't
MeSH terms
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Amino Acid Sequence
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Carbocyanines
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Fluorescent Dyes
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HSP27 Heat-Shock Proteins
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Heat-Shock Proteins / chemistry
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Heat-Shock Proteins / immunology
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Heat-Shock Proteins / isolation & purification
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Humans
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Immunoblotting / methods*
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Immunochemistry
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Molecular Chaperones
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Myocardium / chemistry
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Neoplasm Proteins / chemistry
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Neoplasm Proteins / immunology
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Neoplasm Proteins / isolation & purification
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Peptide Fragments / chemistry
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Peptide Fragments / immunology
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Peptide Fragments / isolation & purification
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Protein Array Analysis / methods
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Proteins / immunology
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Proteins / isolation & purification
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Proteomics / methods*
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Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization
Substances
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Carbocyanines
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Fluorescent Dyes
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HSP27 Heat-Shock Proteins
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HSPB1 protein, human
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Heat-Shock Proteins
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Molecular Chaperones
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Neoplasm Proteins
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Peptide Fragments
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Proteins
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cyanine dye 3
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cyanine dye 5