Cytokine gene variants and venous thrombotic risk in the BRATROS (BRAZILIAN THROMBOSIS STUDY)

Thromb Res. 2007;120(2):221-9. doi: 10.1016/j.thromres.2006.09.015. Epub 2006 Nov 17.

Abstract

Introduction: Venous thrombosis (VT) and inflammation are two closely related entities. In the present investigation we assessed whether there is a relation between genetic modifiers of the inflammatory response and the risk of VT.

Materials and methods: 420 consecutive and unrelated patients with an objective diagnosis of deep VT and 420 matched controls were investigated. The frequencies of the following gene polymorphisms were determined in all subjects: TNF-alpha-308 G/A, LT-alpha+252 A/G, IL-6-174 G/C, IL1-ra 86 bp VNTR, IL-10-1082 A/G and CD-31 125 C/G.

Results: Overall odds ratio (OR) for VT related to TNF-alpha-308 G/A, LT-alpha+252 A/G, IL-6-174 G/C, A1 allele (4 bp repeat) of the IL1-ra 86 bp VNTR, IL-10-1082 A/G and CD-31 125 C/G were respectively: 1.0 (CI95: 0.8-1.5), 1.3 (CI95: 1.0-1.7), 1.1 (CI95: 0.9-1.5), 1.6 (CI95: 1-2.5), 1.2 (CI95: 0.8-1.7) and 0.8 (CI95: 0.6-1.1). A possible interaction between polymorphisms was observed only for the co-inheritance of the mutant alleles of the LT-alpha+252 A/G and IL-10-1082 G/A polymorphisms (OR=2; CI95: 1.1-3.8). The risk of VT conferred by factor V Leiden and FII G20210A was not substantially altered by co-inheritance with any of the cytokine gene polymorphisms.

Conclusions: Cytokine gene polymorphisms here investigated did not significantly influence venous thrombotic risk.

MeSH terms

  • Adolescent
  • Adult
  • Aged
  • Alleles
  • Base Sequence
  • Brazil
  • Case-Control Studies
  • Child
  • Child, Preschool
  • Cytokines / genetics*
  • DNA Primers / genetics
  • Female
  • Gene Frequency
  • Genetic Variation*
  • Humans
  • Interleukin 1 Receptor Antagonist Protein / genetics
  • Interleukin-10 / genetics
  • Interleukin-6 / genetics
  • Lymphotoxin-alpha / genetics
  • Male
  • Middle Aged
  • Odds Ratio
  • Platelet Endothelial Cell Adhesion Molecule-1 / genetics
  • Polymorphism, Genetic
  • Risk Factors
  • Tumor Necrosis Factor-alpha / genetics
  • Venous Thrombosis / etiology
  • Venous Thrombosis / genetics*
  • Venous Thrombosis / immunology*

Substances

  • Cytokines
  • DNA Primers
  • IL10 protein, human
  • IL6 protein, human
  • Interleukin 1 Receptor Antagonist Protein
  • Interleukin-6
  • Lymphotoxin-alpha
  • Platelet Endothelial Cell Adhesion Molecule-1
  • Tumor Necrosis Factor-alpha
  • Interleukin-10