Electronmicroscopic detection of the axonal coexistence of serotonin and substance P in B1-B2 raphé cells transplanted into the transected spinal cord of adult rats

Brain Res. 1991 Feb 22;542(1):159-62. doi: 10.1016/0006-8993(91)91012-p.

Abstract

One week after a complete spinal cord transection at the thoracic (T8) level in adult rats, a suspension of rhombencephalic embryonic (day 14) cells containing the B1-B2 serotonergic groups was injected below the section. After a survival period of one month, the spinal cord was processed for an ultrastructural dual immunocytochemical detection of serotonin (5-HT) and substance P (SP). It was shown by ultrastructural dual immunolabeling that 5-HT and SP coexist in the same axon terminals of transplanted cells.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Animals
  • Axons / metabolism*
  • Axons / ultrastructure
  • Denervation
  • Fetal Tissue Transplantation
  • Immunohistochemistry
  • Microscopy, Electron
  • Nerve Tissue / transplantation*
  • Raphe Nuclei / embryology
  • Raphe Nuclei / metabolism*
  • Raphe Nuclei / ultrastructure
  • Rats
  • Rats, Inbred Strains
  • Serotonin / metabolism*
  • Spinal Cord / physiology*
  • Spinal Cord / ultrastructure
  • Substance P / metabolism*
  • Tissue Distribution

Substances

  • Serotonin
  • Substance P