Hybridization properties of deoxyoligonucleotides containing anthraquinone pseudonucleosides

Nucleic Acids Res. 1991 Jun 11;19(11):3111-4. doi: 10.1093/nar/19.11.3111.

Abstract

Achiral pseudonucleosides bearing an anthraquinone moiety have been incorporated into deoxyoligonucleotides in internal, 3' and 5' positions. The ability of these modified deoxyoligonucleotides to hybridize to complementary RNA and DNA was investigated using Tm measurements. The anthraquinone was shown to enhance binding to a complementary RNA when linked to the 3' and 5' end. Pseudonucleoside substitutions on the 3' end of an oligonucleotide are also shown to confer serum stability to the oligonucleotide.

MeSH terms

  • Anthraquinones / chemistry*
  • Base Sequence
  • DNA / genetics
  • Deoxyribonucleotides / chemistry
  • Deoxyribonucleotides / genetics*
  • Molecular Sequence Data
  • Nucleic Acid Hybridization
  • Nucleosides / chemistry*
  • Phosphorylation
  • RNA / genetics

Substances

  • Anthraquinones
  • Deoxyribonucleotides
  • Nucleosides
  • RNA
  • DNA