Abstract
Since drug-activated gammadelta T cells promote dendritic cell (DC) maturation, we analyzed the effect of combining gammadelta T cell specific drugs with BCG in vitro. BCG-induced DC maturation was increased by bromohydrin-pirophosphate (BrHPP) or zoledronate (Zol)-activated gammadelta T cells. Specifically, the co-culture with activated Vgamma9Vdelta2 T cells with BCG-infected DC resulted in a significant increase of the expression of CD80, CD86, CD40 and CD25 molecules on DC. Moreover, DC were able to produce increased levels of TNF-alpha and synthesize ex novo IL-15 without altering the IL-10/IL-12 immunoregulatory pathway. Finally, the Th1 immunity induced by BCG-infected DC on naïve CD4 T cells was increased by gammadelta T cell activation with BrHpp or Zol. These data indicate that gammadelta T cell triggering drugs could be used to enhance the BCG induced Th1 immunity.
Publication types
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Research Support, Non-U.S. Gov't
MeSH terms
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Alcohols / pharmacology*
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CD4-Positive T-Lymphocytes / immunology
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Coculture Techniques
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Dendritic Cells / drug effects
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Dendritic Cells / immunology*
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Dendritic Cells / microbiology
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Diphosphonates / pharmacology*
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Humans
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Imidazoles / pharmacology*
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Interleukin-10 / immunology
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Interleukin-15 / immunology
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Lymphocyte Activation
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Mycobacterium bovis / immunology*
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Mycobacterium tuberculosis / immunology
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Receptors, Antigen, T-Cell, gamma-delta / immunology*
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Th1 Cells / drug effects
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Th1 Cells / immunology*
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Tuberculosis Vaccines / immunology
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Tuberculosis Vaccines / therapeutic use
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Tumor Necrosis Factor-alpha / biosynthesis
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Tumor Necrosis Factor-alpha / immunology
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Zoledronic Acid
Substances
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Alcohols
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Diphosphonates
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Imidazoles
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Interleukin-15
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Receptors, Antigen, T-Cell, gamma-delta
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Tuberculosis Vaccines
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Tumor Necrosis Factor-alpha
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bromohydrins
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Interleukin-10
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Zoledronic Acid