Bovine papillomavirus type-2 DNA and expression of E5 and E7 oncoproteins in vascular tumours of the urinary bladder in cattle

Cancer Lett. 2007 May 18;250(1):82-91. doi: 10.1016/j.canlet.2006.09.022. Epub 2006 Nov 27.

Abstract

Cattles suffering from chronic enzootic haematuria frequently develop urinary bladder tumours of both epithelial and mesenchymal origin mainly haemangioma and its malignant counterpart. The role of the bovine papillomavirus type-2 (BPV-2) and of its major transforming oncoprotein in naturally occurring urothelial carcinogenesis has been recently clarified. E5 interacts in vivo as in vitro with the beta receptor for the platelet-derived growth factor (PDGF). However, studies regarding tumours of mesenchymal origin such as those arising from blood vessels are lacking. We show that the BPV-2 is present in 100% of the vascular tumours of the urinary bladder examined. Twenty-six out of twenty-seven tumour samples (96%) expressed E5 while 20 out of 27 (74%) tumour samples expressed E7. The two viral oncoproteins were not expressed in normal endothelial cells. Additionally, they co-localize in neoplastic endothelial cells as demonstrated by confocal immunofluorescence. PDGFbeta receptor was also shown to be expressed and co-localizes with E5 in neoplastic blood vessels. Our results demonstrate, for the first time, that the BPV-2 is present in high percentage in tumours of mesenchymal origin arising in its natural host. Furthermore, the expression of the two viral oncoproteins confirm that the virus may have a causative role in the neoplastic process.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Animals
  • Bovine papillomavirus 1 / genetics*
  • Cattle
  • Cattle Diseases
  • DNA, Viral / metabolism*
  • Oncogene Proteins / metabolism
  • Oncogene Proteins, Viral
  • Papillomavirus E7 Proteins
  • Receptor, Platelet-Derived Growth Factor beta / metabolism
  • Urinary Bladder Neoplasms / veterinary*
  • Urinary Bladder Neoplasms / virology*
  • Vascular Neoplasms / genetics*
  • Vascular Neoplasms / virology*

Substances

  • DNA, Viral
  • Oncogene Proteins
  • Oncogene Proteins, Viral
  • Papillomavirus E7 Proteins
  • oncogene protein E7, Human papillomavirus type 16
  • Receptor, Platelet-Derived Growth Factor beta