Abstract
Mouse anti-Fas monoclonal antibody has a cytolytic activity on human cells that express the antigen. Complementary DNAs encoding the cell surface antigen Fas were isolated from a cDNA library of human T cell lymphoma KT-3 cells. The nucleotide sequence of the cDNAs revealed that the molecule coding for the Fas antigen determinant is a 319 amino acid polypeptide (Mr 36,000) with a single transmembrane domain. The extracellular domain is rich in cysteine residue, and shows a similarity to that of human tumor necrosis factor receptors, human nerve growth factor receptor, and human B cell antigen CD40. Murine WR19L cells or L929 cells transformed with the human Fas antigen cDNA were killed by the anti-Fas antibody in the process known as apoptosis.
Publication types
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Comparative Study
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Research Support, Non-U.S. Gov't
MeSH terms
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Amino Acid Sequence
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Animals
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Antigens, Surface / genetics
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Antigens, Surface / physiology*
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Base Sequence
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Blotting, Northern
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Cell Line
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Cell Survival*
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DNA, Neoplasm / genetics
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DNA, Neoplasm / isolation & purification
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Gene Expression
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Gene Library
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Humans
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L Cells / ultrastructure
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Mice
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Molecular Sequence Data
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Nucleic Acid Hybridization
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Protein Conformation
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RNA, Messenger / analysis
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RNA, Messenger / genetics
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Recombinant Proteins / metabolism
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Restriction Mapping
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Sequence Homology, Nucleic Acid
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Transfection
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fas Receptor
Substances
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Antigens, Surface
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DNA, Neoplasm
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RNA, Messenger
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Recombinant Proteins
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fas Receptor
Associated data
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GENBANK/M60962
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GENBANK/M64383
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GENBANK/M64384
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GENBANK/M67454
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GENBANK/M74093
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GENBANK/S44028
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GENBANK/S56134
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GENBANK/S56136
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GENBANK/S56137
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GENBANK/S56139