Abstract
In order to establish whether endothelial cells are involved in immunodeficiency virus type 1 (HIV-1) infection, we performed a virological study on endothelial cells isolated from human adipose tissue and infected with HIV-1 in vitro. Supernatants from cultures showed a reverse transcriptase activity starting one day after HIV inoculation. Viral rescue was significantly impaired in cycloheximide treated cells confirming a de novo synthesis of viral products.
Publication types
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Research Support, Non-U.S. Gov't
MeSH terms
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Adipose Tissue
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Cells, Cultured
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Cycloheximide / pharmacology
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Endothelium / cytology
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Endothelium / microbiology*
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Gene Products, gag / analysis
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HIV Antigens / analysis
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HIV Core Protein p24
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HIV-1 / enzymology
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HIV-1 / immunology
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HIV-1 / physiology*
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Humans
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RNA-Directed DNA Polymerase / analysis
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Viral Core Proteins / analysis
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Virus Replication
Substances
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Gene Products, gag
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HIV Antigens
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HIV Core Protein p24
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Viral Core Proteins
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Cycloheximide
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RNA-Directed DNA Polymerase