Abstract
In order to investigate whether the leukemic B cells in B-CLL express immunoglobulin genes in mutated or unmutated form, independent cDNA clones of one patient with B-CLL expressing heavy and light chain V region genes were sequenced. The sequences of both the VH and V kappa clones were identical. The V genes could be assigned to known germline V genes. No somatic mutations were found. At the V kappa-J kappa border there is an insertion of N-sequences which are only rarely found in immunoglobulin light chain genes. Our study confirms other published data on V gene expression in B-CLL in that the surface immunoglobulins in these tumors are unmutated.
Publication types
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Comparative Study
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Research Support, Non-U.S. Gov't
MeSH terms
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Antigens, Differentiation / analysis
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Antigens, Surface / analysis
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B-Lymphocytes / immunology
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B-Lymphocytes / physiology
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Base Sequence
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Blotting, Southern
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CD5 Antigens
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DNA, Neoplasm / genetics
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Female
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Gene Expression
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Humans
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Immunoglobulin Heavy Chains / genetics*
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Immunoglobulin Light Chains / genetics*
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Immunoglobulin M / genetics
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Leukemia, Lymphocytic, Chronic, B-Cell / genetics*
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Leukemia, Lymphocytic, Chronic, B-Cell / pathology
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Male
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Middle Aged
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Molecular Sequence Data
Substances
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Antigens, Differentiation
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Antigens, Surface
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CD5 Antigens
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DNA, Neoplasm
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Immunoglobulin Heavy Chains
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Immunoglobulin Light Chains
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Immunoglobulin M