Abstract
Background:
Androgen-independent prostate adenocarcinomas are responsible for about 6% of overall cancer deaths in men.
Methods:
We used DNA microarrays to identify genes related to the transition between androgen-dependent and androgen-independent stages in the LuCaP 23.1 xenograft model of prostate adenocarcinoma. The expression of the proteins encoded by these genes was then assessed by immunohistochemistry on tissue microarrays (TMA) including human prostate carcinoma samples issued from 85 patients who had undergone radical prostatectomy.
Results:
FGFR1, TACC1 and WT1 gene expression levels were associated with the androgen-independent stage in xenografts and human prostate carcinoma samples. MART1 protein expression was correlated with pT2 tumor stages.
Conclusion:
Our results suggest that each of these four genes may play a role, or at least reflect a stage of prostate carcinoma growth/development/progression.
Publication types
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Research Support, Non-U.S. Gov't
MeSH terms
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Adenocarcinoma / metabolism*
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Adenocarcinoma / pathology*
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Animals
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Antigens, Neoplasm / metabolism
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Biomarkers, Tumor / isolation & purification*
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Biomarkers, Tumor / metabolism
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Disease Progression
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Fetal Proteins / metabolism
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Gene Expression Regulation, Neoplastic
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Humans
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Immunohistochemistry
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MART-1 Antigen
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Male
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Mice
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Microtubule-Associated Proteins / metabolism
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Middle Aged
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Models, Biological
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Neoplasm Proteins / metabolism
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Neoplasm Staging / methods
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Nuclear Proteins / metabolism
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Oligonucleotide Array Sequence Analysis
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Prostatic Neoplasms / metabolism*
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Prostatic Neoplasms / pathology*
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Receptor, Fibroblast Growth Factor, Type 1 / metabolism*
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WT1 Proteins / metabolism*
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Xenograft Model Antitumor Assays
Substances
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Antigens, Neoplasm
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Biomarkers, Tumor
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Fetal Proteins
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MART-1 Antigen
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MLANA protein, human
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Microtubule-Associated Proteins
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Mlana protein, mouse
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Neoplasm Proteins
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Nuclear Proteins
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TACC1 protein, human
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WT1 Proteins
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Receptor, Fibroblast Growth Factor, Type 1