One-step generation of recombineering constructs by asymmetric-end ligation and negative selection

Anal Biochem. 2007 Jan 15;360(2):306-8. doi: 10.1016/j.ab.2006.05.001. Epub 2006 May 19.

Authors

Masato Ohtsuka¹, Akiko Mizutani, Yukie Y Kikuti, Jerzy K Kulski, Masahiro Sato, Minoru Kimura, Masafumi Tanaka, Hidetoshi Inoko

Affiliation

¹ Division of Basic Molecular Science and Molecular Medicine, School of Medicine, Tokai University, Bohseidai, Isehara, Kanagawa 259-1193, Japan. [email protected]

PMID: 17141172
DOI: 10.1016/j.ab.2006.05.001

No abstract available

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Cloning, Molecular
Genetic Engineering / methods*
Genetic Vectors / genetics
Green Fluorescent Proteins / genetics
Green Fluorescent Proteins / metabolism
Polymerase Chain Reaction
Recombinant Fusion Proteins / genetics
Recombinant Fusion Proteins / metabolism
Recombination, Genetic / genetics*

Substances

Recombinant Fusion Proteins
Green Fluorescent Proteins