Screening of modified DNA aptamers that recognize DNA secondary structure

Nucleic Acids Symp Ser (Oxf). 2004:(48):265-6. doi: 10.1093/nass/48.1.265.

Abstract

We attempted to utilize in vitro selection techniques to develop modified DNA aptamers that are specific for DNA secondary structure including a single-mismatch base pair. For the selection, two kinds of random modified DNA library were prepared by an enzymatic method using modified 2'-deoxynucleoside 5'-triphosphates and KOD Dash DNA polymerase: one library consists of modified DNA including amino functionality and another consists of modified DNA including both hydroxypropynyl and guanidium functionalities.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Aptamers, Nucleotide / isolation & purification*
  • Base Pair Mismatch
  • Base Sequence
  • DNA / chemistry*
  • DNA-Directed DNA Polymerase / metabolism
  • Gene Library
  • Molecular Sequence Data
  • Nucleic Acid Conformation*
  • SELEX Aptamer Technique / methods*

Substances

  • Aptamers, Nucleotide
  • DNA
  • DNA-Directed DNA Polymerase