SNPs analysis by use of oligonucleotides containing 2'-O-methyl-2-thiouridines

Itaru Okamoto; Kohji Seio; Mitsuo Sekine

doi:10.1093/nass/49.1.123

SNPs analysis by use of oligonucleotides containing 2'-O-methyl-2-thiouridines

Nucleic Acids Symp Ser (Oxf). 2005:(49):123-4. doi: 10.1093/nass/49.1.123.

Authors

Itaru Okamoto¹, Kohji Seio, Mitsuo Sekine

Affiliation

¹ Graduate School of Life Science, Tokyo Institute of Technology.

PMID: 17150664
DOI: 10.1093/nass/49.1.123

Abstract

In this study, we report an improved method for the synthesis of oligonucleotides containing 2-thiouridine (s2U) derivatives. The hybridization affinity and base pair recognition ability of oligonucleotides having 2-thiouracil moieties with both the complementary RNAs and DNAs were also studied in detail. These results indicate that oligonucleotides containing s2U derivatives could be used as powerful tools for chip-based SNPs detection.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Base Pairing
Oligonucleotide Array Sequence Analysis*
Oligonucleotide Probes / chemical synthesis
Oligonucleotide Probes / chemistry*
Polymorphism, Single Nucleotide*
Thiouridine / analogs & derivatives*
Thiouridine / chemistry

Substances

2'-O-methyl-2-thiouridine
Oligonucleotide Probes
Thiouridine