Design, synthesis and evaluation of oligomer conjugated MGBpolyamide-nucleoside hybrid as a novel gene expression control compound

Etsuko Kawashima; Yusuke Ohba; Yusuke Terui; Kazuo Kamaike

doi:10.1093/nass/49.1.327

Design, synthesis and evaluation of oligomer conjugated MGBpolyamide-nucleoside hybrid as a novel gene expression control compound

Nucleic Acids Symp Ser (Oxf). 2005:(49):327-8. doi: 10.1093/nass/49.1.327.

Authors

Etsuko Kawashima¹, Yusuke Ohba, Yusuke Terui, Kazuo Kamaike

Affiliation

¹ School of Pharmacy, Tokyo University of Pharmacy and Life Science, 1432-1 Horinouchi, Hachioji, Tokyo 192-0392, Japan.

PMID: 17150766
DOI: 10.1093/nass/49.1.327

Abstract

DNA oligomers conjugated pyrrolepolyamide (minor groove binder)-deoxyguanosine hybrid were synthesized as novel gene expression control compounds. From T(m) values and CD spectral analysis, it was found that oligomers conjugated hybrid possess high recognition ability and very high binding ability for the DNA that includes pyrrolepolyamide match site.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Circular Dichroism
DNA / chemistry
Deoxyguanosine / analogs & derivatives*
Deoxyguanosine / chemical synthesis
Deoxyguanosine / chemistry
Gene Expression Regulation
Genetic Therapy
Oligodeoxyribonucleotides, Antisense / chemistry*
Pyrroles / chemical synthesis
Pyrroles / chemistry*
Temperature

Substances

2'-deoxy-N(2)-(3-(4-(4-(4-formamido-1-methylpyrrole-2-carboxamido)-1-methylpyrrole-2-carboxamido)-1-methylpyrrole-2-carboxamido)propyl)guanosine
Oligodeoxyribonucleotides, Antisense
Pyrroles
DNA
Deoxyguanosine