Oligomeric structure, dynamics, and orientation of membrane proteins from solid-state NMR

Structure. 2006 Dec;14(12):1731-40. doi: 10.1016/j.str.2006.10.002.

Abstract

Solid-state NMR is a versatile and powerful tool for determining the dynamic structure of membrane proteins at atomic resolution. I review the recent progress in determining the orientation, the internal and global protein dynamics, the oligomeric structure, and the ligand-bound structure of membrane proteins with both alpha-helical and beta sheet conformations. Examples are given that illustrate the insights into protein function that can be gained from the NMR structural information.

Publication types

  • Research Support, N.I.H., Extramural
  • Research Support, U.S. Gov't, Non-P.H.S.
  • Review

MeSH terms

  • Animals
  • Anisotropy
  • Cell Membrane / metabolism
  • Dose-Response Relationship, Drug
  • Humans
  • Ligands
  • Magnetic Resonance Spectroscopy / methods*
  • Membrane Proteins / chemistry
  • Membrane Proteins / physiology*
  • Models, Molecular
  • Peptides / chemistry
  • Potassium / chemistry
  • Potassium Channels / chemistry
  • Protein Conformation
  • Protein Structure, Secondary
  • Proteins / chemistry

Substances

  • Ligands
  • Membrane Proteins
  • Peptides
  • Potassium Channels
  • Proteins
  • Potassium