Diagnostic utility of FLI-1 monoclonal antibody and dual-colour, break-apart probe fluorescence in situ (FISH) analysis in Ewing's sarcoma/primitive neuroectodermal tumour (EWS/PNET). A comparative study with CD99 and FLI-1 polyclonal antibodies

Histopathology. 2006 Dec;49(6):569-75. doi: 10.1111/j.1365-2559.2006.02535.x.

Abstract

Aims: To compare the sensitivity and specificity of the recently commercially available FLI-1 monoclonal (FLI-1m) antibody with the currently used antibodies [CD99 and FLI-1 polyclonal (FLI-1p)] in the diagnosis of Ewing's sarcoma/primitive neuroectodermal tumour (EWS/PNET) and to determine the diagnostic value of the EWSR1 (22q12) dual-colour, break-apart rearrangement probe fluorescence in situ hybridization (FISH) technique.

Materials and methods: Forty-three cases of well-documented EWS/PNET and 15 non-EWS/PNET cases were retrieved from the archival files. Immunohistochemistry (IHC) for FLI-1p, FLI-1m and FISH analysis was performed.

Results: The most sensitive and specific test panel for the diagnosis of EWS/PNET is the combination of CD99 and FLI-1p. FISH had a very high specificity (100%) but only a moderate sensitivity (50%).

Conclusion: The combination of CD99 and FLI-1p is the method of choice for the diagnosis of EWS/PNET. EWRS1 (22q12) dual-colour, break-apart rearrangement probe FISH should be used as a confirmatory test in addition to CD99 and FLI1-p due to its high specificity.

Publication types

  • Comparative Study

MeSH terms

  • 12E7 Antigen
  • Actins
  • Algorithms
  • Antibodies, Monoclonal / immunology*
  • Antibodies, Neoplasm / immunology
  • Antigens, CD / immunology*
  • Biomarkers, Tumor / analysis
  • Biomarkers, Tumor / genetics
  • Bone Neoplasms / diagnosis*
  • Bone Neoplasms / genetics
  • Bone Neoplasms / immunology
  • Cell Adhesion Molecules / immunology*
  • Chromosome Aberrations
  • Female
  • Humans
  • In Situ Hybridization, Fluorescence / methods
  • Male
  • Microfilament Proteins / genetics*
  • Microfilament Proteins / immunology*
  • Neoplasm Proteins / immunology
  • Neuroectodermal Tumors, Primitive / diagnosis*
  • Neuroectodermal Tumors, Primitive / genetics
  • Neuroectodermal Tumors, Primitive / immunology
  • Predictive Value of Tests
  • ROC Curve
  • Receptors, Cytoplasmic and Nuclear / genetics*
  • Receptors, Cytoplasmic and Nuclear / immunology*
  • Sarcoma, Ewing / diagnosis*
  • Sarcoma, Ewing / genetics
  • Sarcoma, Ewing / immunology
  • Trans-Activators

Substances

  • 12E7 Antigen
  • Actins
  • Antibodies, Monoclonal
  • Antibodies, Neoplasm
  • Antigens, CD
  • Biomarkers, Tumor
  • CD99 protein, human
  • Cell Adhesion Molecules
  • FLII protein, human
  • Microfilament Proteins
  • Neoplasm Proteins
  • Receptors, Cytoplasmic and Nuclear
  • Trans-Activators