Detection of hepatitis C virus infection by enzyme-linked immunosorbent assay system using core protein expressed in Escherichia coli

K Muraiso; M Hijikata; N Kato; K Shimotohno; N Okazaki; S Ohkoshi; M Uura; S Kaneko; K Kobayashi; M Omata

doi:10.1111/j.1349-7006.1991.tb01914.x

Detection of hepatitis C virus infection by enzyme-linked immunosorbent assay system using core protein expressed in Escherichia coli

Jpn J Cancer Res. 1991 Aug;82(8):879-82. doi: 10.1111/j.1349-7006.1991.tb01914.x.

Authors

K Muraiso¹, M Hijikata, N Kato, K Shimotohno, N Okazaki, S Ohkoshi, M Uura, S Kaneko, K Kobayashi, M Omata, et al.

Affiliation

¹ Virology Division, National Cancer Center Research Institute, Tokyo.

Abstract

Enzyme-linked immunosorbent assay of the core protein of hepatitis C virus (HCV) expressed in E. coli led to detection of the antibody against this virus in patients with chronic hepatitis. Some of the negative results obtained using a different viral protein became positive with this E. coli-expressed viral protein, and were also positive for the viral RNA. Thus, use of the core protein of HCV facilitates accurate detection of HCV infection.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Base Sequence
Enzyme-Linked Immunosorbent Assay
Escherichia coli / genetics*
Hepatitis Antibodies / analysis
Hepatitis C / diagnosis*
Hepatitis C Antibodies
Humans
Molecular Sequence Data
RNA, Viral / analysis
Serologic Tests
Viral Core Proteins / analysis
Viral Core Proteins / immunology*

Substances

Hepatitis Antibodies
Hepatitis C Antibodies
RNA, Viral
Viral Core Proteins