Positive correlation between pigment epithelium-derived factor and monocyte chemoattractant protein-1 levels in the aqueous humour of patients with uveitis

Br J Ophthalmol. 2007 Jun;91(6):737-8. doi: 10.1136/bjo.2006.109843. Epub 2006 Dec 13.

Abstract

Aim: To evaluate whether aqueous humour levels of pigment epithelium-derived factor (PEDF) are associated with monocyte chemoattractant protein-1 (MCP-1) in patients with uveitis.

Methods: Aqueous humour levels of MCP-1 and PEDF were determined by ELISA in 34 uveitis samples and 9 cataract control samples.

Results: Aqueous humour MCP-1 and PEDF levels were significantly higher in patients with infectious or non-infectious uveitis than in controls (mean (SD) 32.3 (10.7) ng/ml vs 4.48 (1.10) ng/ml vs 0.47 (0.10) ng/ml, and 8.40 (1.30) microg/ml vs 5.01 (0.92) microg/ml vs 1.32 (0.22) microg/ml, respectively, p<0.001). A positive correlation between PEDF and MCP-1 was found in patients with uveitis (r = 0.39, p<0.01).

Conclusion: The results demonstrated that aqueous humour levels of PEDF were positively associated with MCP-1 in patients with uveitis. The present observations suggest that aqueous humour levels of PEDF may be a marker of inflammation in uveitis.

MeSH terms

  • Aged
  • Aqueous Humor / chemistry*
  • Biomarkers / analysis
  • Cataract / metabolism
  • Chemokine CCL2 / analysis*
  • Enzyme-Linked Immunosorbent Assay / methods
  • Eye Infections / metabolism
  • Eye Proteins / analysis*
  • Humans
  • Middle Aged
  • Nerve Growth Factors / analysis*
  • Serpins / analysis*
  • Uveitis / metabolism*

Substances

  • Biomarkers
  • Chemokine CCL2
  • Eye Proteins
  • Nerve Growth Factors
  • Serpins
  • pigment epithelium-derived factor