KIN-29 SIK regulates chemoreceptor gene expression via an MEF2 transcription factor and a class II HDAC

EMBO J. 2007 Jan 24;26(2):358-70. doi: 10.1038/sj.emboj.7601479. Epub 2006 Dec 14.

Abstract

The expression of individual chemoreceptor (CR) genes in Caenorhabditis elegans is regulated by multiple environmental and developmental cues, possibly enabling C. elegans to modulate its sensory responses. We had previously shown that KIN-29, a member of the salt-inducible kinase family, acts in a subset of chemosensory neurons to regulate the expression of CR genes, body size and entry into the alternate dauer developmental stage. Here, we show that KIN-29 regulates these processes by phosphorylating the HDA-4 class II histone deacetylase (HDAC) and inhibiting the gene repression functions of HDA-4 and an MEF-2 MADS domain transcription factor. MEF-2 binds directly to the CR gene regulatory sequences, and is required only to repress but not activate CR gene expression. A calcineurin phosphatase antagonizes the KIN-29/MEF-2-regulated pathway to modulate levels of CR gene expression. Our results identify KIN-29 as a new regulator of MEF2/HDAC functions in the nervous system, reveal cell-specific mechanisms of action of this pathway in vivo and demonstrate remarkable complexity in the regulation of CR gene expression in C. elegans.

Publication types

  • Research Support, N.I.H., Extramural
  • Research Support, U.S. Gov't, Non-P.H.S.

MeSH terms

  • Animals
  • Animals, Genetically Modified
  • Binding Sites
  • Caenorhabditis elegans
  • Caenorhabditis elegans Proteins / genetics
  • Caenorhabditis elegans Proteins / physiology*
  • Calcineurin / physiology
  • Calcium Signaling
  • Chemoreceptor Cells / metabolism*
  • Gene Expression Regulation
  • Histone Deacetylases / genetics
  • Histone Deacetylases / physiology*
  • MADS Domain Proteins / genetics
  • MADS Domain Proteins / physiology*
  • Models, Biological
  • Mutation
  • Protein Binding
  • Protein Serine-Threonine Kinases / genetics
  • Protein Serine-Threonine Kinases / metabolism
  • Protein Serine-Threonine Kinases / physiology*
  • Transcription Factors / genetics
  • Transcription Factors / physiology*

Substances

  • Caenorhabditis elegans Proteins
  • MADS Domain Proteins
  • Transcription Factors
  • mef-2 protein, C elegans
  • kin-29 protein, C elegans
  • Protein Serine-Threonine Kinases
  • Calcineurin
  • HDA-4 protein, C elegans
  • Histone Deacetylases