Rice XA21 binding protein 3 is a ubiquitin ligase required for full Xa21-mediated disease resistance

Yong-Sheng Wang; Li-Ya Pi; Xiuhua Chen; Pranjib K Chakrabarty; Junda Jiang; Alfred Lopez De Leon; Guo-Zhen Liu; Liangcai Li; Ulla Benny; James Oard; Pamela C Ronald; Wen-Yuan Song

doi:10.1105/tpc.106.046730

Rice XA21 binding protein 3 is a ubiquitin ligase required for full Xa21-mediated disease resistance

Plant Cell. 2006 Dec;18(12):3635-46. doi: 10.1105/tpc.106.046730. Epub 2006 Dec 15.

Authors

Yong-Sheng Wang¹, Li-Ya Pi, Xiuhua Chen, Pranjib K Chakrabarty, Junda Jiang, Alfred Lopez De Leon, Guo-Zhen Liu, Liangcai Li, Ulla Benny, James Oard, Pamela C Ronald, Wen-Yuan Song

Affiliation

¹ Department of Plant Pathology, University of Florida, Gainesville, Florida 32611, USA.

Abstract

XA21 is a receptor-like kinase protein in rice (Oryza sativa) that confers gene-for-gene resistance to specific races of the causal agent of bacterial blight disease, Xanthomonas oryzae pv oryzae. We identified XA21 binding protein 3 (XB3), an E3 ubiquitin ligase, as a substrate for the XA21 Ser and Thr kinase. The interaction between XB3 and the kinase domain of XA21 has been shown in yeast and in vitro, and the physical association between XB3 and XA21 in vivo has also been confirmed by coimmunoprecipitation assays. XB3 contains an ankyrin repeat domain and a RING finger motif that is sufficient for its interaction with the kinase domain of XA21 and for its E3 ubiquitin ligase activity, respectively. Transgenic plants with reduced expression of the Xb3 gene are compromised in resistance to the avirulent race of X. oryzae pv oryzae. Furthermore, reduced levels of Xb3 lead to decreased levels of the XA21 protein. These results indicate that Xb3 is necessary for full accumulation of the XA21 protein and for Xa21-mediated resistance.

Publication types

Research Support, N.I.H., Extramural
Research Support, U.S. Gov't, Non-P.H.S.

MeSH terms

Amino Acid Sequence
Ankyrin Repeat
Gene Dosage
Gene Expression Regulation, Enzymologic
Gene Expression Regulation, Plant
Immunity, Innate / genetics
Immunity, Innate / immunology*
Molecular Sequence Data
Oryza / enzymology*
Oryza / genetics
Phosphorylation
Plant Diseases / genetics
Plant Diseases / immunology*
Plant Proteins / chemistry
Plant Proteins / genetics
Plant Proteins / metabolism*
Protein Binding
Protein Serine-Threonine Kinases / metabolism*
Protein Structure, Tertiary
RNA, Messenger / genetics
RNA, Messenger / metabolism
Recombinant Fusion Proteins / metabolism
Substrate Specificity
Thermodynamics
Ubiquitin / metabolism
Ubiquitin-Protein Ligases / chemistry
Ubiquitin-Protein Ligases / genetics
Ubiquitin-Protein Ligases / metabolism*
Yeasts / enzymology

Substances

Plant Proteins
RNA, Messenger
Recombinant Fusion Proteins
Ubiquitin
Xa21 protein, Oryza sativa
Ubiquitin-Protein Ligases
Protein Serine-Threonine Kinases

Associated data

GENBANK/AF272860
GENBANK/AK059792
GENBANK/AK065223
GENBANK/AK067289
GENBANK/AK106014
GENBANK/AK120632
GENBANK/DQ088999