Abstract
The development of rapid and sensitive molecular techniques for the detection of Vibrio species would be useful for the surveillance of sporadic infections and management of major outbreaks. Comparative sequence analysis of the ftsZ gene in the predominant Vibrio species that cause human disease revealed distinct alleles for each examined species, including Vibrio cholerae, Vibrio parahaemolyticus and Vibrio vulnificus. Light Upon eXtension (LUX) real-time PCR assays were developed to target these species-specific polymorphisms, and were successful in rapidly differentiating the major pathogenic Vibrio species. Luminex liquid microsphere array technology was used to develop a comprehensive assay capable of simultaneously detecting V. cholerae, V. parahaemolyticus and V. vulnificus. These assays permitted the identification of a presumptive V. parahaemolyticus isolate as Vibrio alginolyticus, which was verified using additional molecular characterization.
MeSH terms
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Bacterial Proteins / genetics*
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Bacterial Typing Techniques / instrumentation
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Bacterial Typing Techniques / methods*
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Base Sequence
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Cytoskeletal Proteins / genetics*
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DNA, Bacterial / chemistry
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DNA, Bacterial / isolation & purification
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Genetic Variation
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Microspheres
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Molecular Sequence Data
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Phylogeny
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Polymerase Chain Reaction / methods*
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Polymorphism, Genetic
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Reproducibility of Results
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Sequence Analysis, DNA
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Sequence Homology, Nucleic Acid
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Species Specificity
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Vibrio / classification
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Vibrio / genetics*
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Vibrio cholerae / genetics
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Vibrio parahaemolyticus / genetics
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Vibrio vulnificus / genetics
Substances
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Bacterial Proteins
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Cytoskeletal Proteins
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DNA, Bacterial
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FtsZ protein, Bacteria
Associated data
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GENBANK/DQ520257
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GENBANK/DQ520258
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GENBANK/DQ520259
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GENBANK/DQ520260
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GENBANK/DQ520261
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GENBANK/DQ520262
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GENBANK/DQ520263
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GENBANK/DQ520264
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GENBANK/DQ520265
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GENBANK/DQ520266
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GENBANK/DQ520267
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GENBANK/DQ520268
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GENBANK/DQ520269
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GENBANK/DQ520270
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GENBANK/DQ520271
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GENBANK/DQ520272
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GENBANK/DQ520273
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GENBANK/DQ520274
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GENBANK/DQ520275
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GENBANK/DQ520276