Determination of clarithromycin in human plasma by liquid chromatography-electrospray ionization tandem mass spectrometry

J Pharm Biomed Anal. 2007 Mar 12;43(4):1460-4. doi: 10.1016/j.jpba.2006.11.025. Epub 2006 Dec 15.

Abstract

A rapid and sensitive method has been developed for the determination of clarithromycin in human plasma with liquid chromatography-tandem mass spectrometry. Clarithromycin and the internal standard, telmisartan were precipitated from the matrix (50 microl) with 200 microl acetonitrile and separated by HPLC using formic acid:10 mM ammonium acetate:methanol (1:99:400, v/v/v) as the mobile phase. The assay based on detection by electrospray positive ionization mass spectrometry in the multiple-reaction monitoring mode was finished within 2.4 min. Linearity was over the concentration range 10-5000 ng/ml with a limit of detection of 0.50 ng/ml. Intra- and inter-day precision measured as relative standard deviation were <3.73% and <9.93%, respectively. The method was applied in a bioequivalence study of two tablet formulations of clarithromycin.

MeSH terms

  • Anti-Bacterial Agents / blood*
  • Anti-Bacterial Agents / chemistry
  • Benzimidazoles / chemistry
  • Benzoates / chemistry
  • Chromatography, Liquid / methods*
  • Clarithromycin / blood*
  • Clarithromycin / chemistry
  • Humans
  • Molecular Structure
  • Reproducibility of Results
  • Sensitivity and Specificity
  • Spectrometry, Mass, Electrospray Ionization / methods*
  • Tandem Mass Spectrometry / methods*
  • Telmisartan
  • Time Factors

Substances

  • Anti-Bacterial Agents
  • Benzimidazoles
  • Benzoates
  • Clarithromycin
  • Telmisartan