Studies on the relationship between cell proliferation and cell death: opposite patterns of SGP-2 and ornithine decarboxylase mRNA accumulation in PHA-stimulated human lymphocytes

Biochem Biophys Res Commun. 1991 Oct 15;180(1):59-63. doi: 10.1016/s0006-291x(05)81254-9.

Abstract

To assess the relationship between cell proliferation and cell death, the mRNA accumulation of ornithine decarboxylase (ODC) and sulfated glycoprotein 2 (SGP-2) were measured in human peripheral blood lymphocytes (HPBL) 2-6 hours after stimulation with phytohemagglutinin (PHA). ODC is the rate limiting enzyme of polyamines biosynthesis and its early induction in mitogen-stimulated lymphocytes has been reported. On the other hand, SGP-2, a glycoprotein present in most mammalian tissues, is induced in classical models of apoptosis, such as dexamethasone-treated thymocytes. Indeed, a consistent amount of SGP-2 mRNA in quiescent HPBL, an early and progressive decrease of SGP-2 mRNA and a parallel increase of ODC mRNA accumulation, were observed, in PHA-stimulated HPBL, suggesting that concomitant repression of SGP-2 and induction of ODC genes contribute for the cell entering the cell cycle.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Blotting, Northern
  • Cell Cycle
  • Cell Division
  • Cell Survival
  • Clusterin
  • Electrophoresis, Agar Gel
  • Gene Expression Regulation / genetics
  • Glycoproteins / genetics*
  • Humans
  • Lymphocyte Activation / genetics*
  • Molecular Chaperones*
  • Nucleic Acid Hybridization
  • Ornithine Decarboxylase / genetics*
  • Phytohemagglutinins
  • RNA / analysis
  • RNA, Messenger / metabolism*

Substances

  • CLU protein, human
  • Clusterin
  • Glycoproteins
  • Molecular Chaperones
  • Phytohemagglutinins
  • RNA, Messenger
  • RNA
  • Ornithine Decarboxylase