Preliminary X-ray investigation of a bifunctional inhibitor from Indian finger millet (ragi)

A Srinivasan; A Raman; T P Singh

doi:10.1016/0022-2836(91)90728-o

Preliminary X-ray investigation of a bifunctional inhibitor from Indian finger millet (ragi)

J Mol Biol. 1991 Nov 5;222(1):1-2. doi: 10.1016/0022-2836(91)90728-o.

Authors

A Srinivasan¹, A Raman, T P Singh

Affiliation

¹ Department of Biophysics, All India Institute of Medical Sciences, New Delhi.

PMID: 1719218
DOI: 10.1016/0022-2836(91)90728-o

Abstract

A bifunctional alpha-amylase/trypsin inhibitor that has two binding sites has been purified from ragi. The inhibitor has been crystallized from its ammonium sulphate solution by the vapour diffusion method. The crystals belong to the orthogonal space group P2(1)2(1)2(1) with unit cell dimensions a = 30.49 A, b = 56.30 A, c = 73.65 A and Z = 4.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Amylases / antagonists & inhibitors*
Binding Sites
Cross-Linking Reagents
Poaceae / chemistry*
Trypsin Inhibitors / chemistry*
Trypsin Inhibitors / isolation & purification
X-Ray Diffraction

Substances

Cross-Linking Reagents
Trypsin Inhibitors
Amylases