Induction and characterization of monoclonal anti-idiotypic antibodies reactive with idiotopes of canine parvovirus neutralizing monoclonal antibodies

Vet Immunol Immunopathol. 1991 Aug;29(1-2):139-50. doi: 10.1016/0165-2427(91)90059-l.

Abstract

Monoclonal anti-idiotypic (anti-Id) antibodies (Ab2) were generated against idiotypes (Id) of canine parvovirus (CPV) specific monoclonal antibodies (MoAbs). The binding of most of these anti-Id antibodies to their corresponding Id could be inhibited by antigen, thus classifying these anti-Id antibodies as Ab2 gamma or Ab2 beta. By inhibiting experiments it was shown that these anti-Id antibodies did not recognize interspecies cross-reactive idiotopes, but recognized private idiotopes, uniquely associated with the Id of the anti-CPV MoAb used for immunization. This classifies these anti-Id antibodies as non-internal image Ab2 gamma. The potential use of these non-internal image anti-Id antibodies for the induction of antiviral antibodies in the CPV system is discussed.

MeSH terms

  • Animals
  • Antibodies, Anti-Idiotypic / immunology*
  • Antibodies, Monoclonal / biosynthesis
  • Antibodies, Monoclonal / immunology*
  • Binding, Competitive / immunology
  • Cats
  • Cross Reactions / immunology
  • Dog Diseases / immunology*
  • Dogs
  • Enzyme-Linked Immunosorbent Assay
  • Epitopes / immunology*
  • Immunoglobulin G / analysis
  • Immunoglobulin Idiotypes / immunology
  • Mice
  • Mice, Inbred BALB C
  • Parvoviridae / immunology*
  • Parvoviridae Infections / veterinary*
  • Species Specificity

Substances

  • Antibodies, Anti-Idiotypic
  • Antibodies, Monoclonal
  • Epitopes
  • Immunoglobulin G
  • Immunoglobulin Idiotypes