Abstract
Phosphodiesterase 3B (PDE3B), is known to play an important role in acute insulin and cAMP-mediated regulation of lipid metabolism, and PDE4 are the main PDE types expressed in adipocytes. Here, we show that members of all PDE4 isoforms are expressed in 3T3-L1 and primary mouse adipocytes. Long-term treatment of 3T3-L1 adipocytes with insulin induced up-regulation of PDE3B and PDE4D in a phosphatidylinositol 3-kinase-dependent manner whereas long-term treatment with beta-adrenergic agonists induced down-regulation of PDE3B and up-regulation of PDE4D. Thus, PDE3B and PDE4D can be added to the list of genes regulated by insulin and cAMP-increasing hormones. Altered expression of PDE3B and PDE4D in response to long-term treatment with insulin and catecholamines may contribute to altered regulation of metabolism in diabetes.
Publication types
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Research Support, Non-U.S. Gov't
MeSH terms
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3',5'-Cyclic-AMP Phosphodiesterases / metabolism*
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3T3-L1 Cells
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Adipocytes / cytology
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Adipocytes / drug effects
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Adipocytes / enzymology*
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Adrenergic beta-Agonists / pharmacology
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Animals
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Blotting, Western
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Chromones / pharmacology
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Cyclic Nucleotide Phosphodiesterases, Type 3
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Cyclic Nucleotide Phosphodiesterases, Type 4
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Dioxoles / pharmacology
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Down-Regulation / drug effects
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Enzyme Inhibitors / pharmacology
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Insulin / pharmacology
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Isoproterenol / pharmacology
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Mice
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Morpholines / pharmacology
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Phosphoinositide-3 Kinase Inhibitors
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Time Factors
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Up-Regulation / drug effects
Substances
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Adrenergic beta-Agonists
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Chromones
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Dioxoles
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Enzyme Inhibitors
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Insulin
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Morpholines
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Phosphoinositide-3 Kinase Inhibitors
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disodium (R,R)-5-(2-((2-(3-chlorophenyl)-2-hydroxyethyl)-amino)propyl)-1,3-benzodioxole-2,3-dicarboxylate
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2-(4-morpholinyl)-8-phenyl-4H-1-benzopyran-4-one
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3',5'-Cyclic-AMP Phosphodiesterases
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Cyclic Nucleotide Phosphodiesterases, Type 3
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Cyclic Nucleotide Phosphodiesterases, Type 4
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Pde3b protein, mouse
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Isoproterenol