Detection of minor drug-resistant populations by parallel allele-specific sequencing

Nat Methods. 2007 Feb;4(2):123-5. doi: 10.1038/nmeth995. Epub 2007 Jan 7.

Abstract

We developed a highly sensitive parallel allele-specific sequencing (PASS) assay to simultaneously analyze a large number of viral genomes and detect minor drug-resistant populations at approximately 0.1-0.01% levels. Using this assay on samples from individuals infected with human immunodeficiency viruses (HIV), we successfully detected and quantified minor populations of drug-resistant viruses and performed linkage analysis of multiple-drug resistance mutations. This assay may serve as a useful tool to study drug resistance in HIV and other infectious agents.

Publication types

  • Research Support, N.I.H., Extramural

MeSH terms

  • Anti-HIV Agents / pharmacology*
  • Antiretroviral Therapy, Highly Active
  • Drug Resistance, Viral*
  • Genetic Linkage
  • Genome, Viral*
  • HIV / drug effects*
  • HIV / genetics*
  • HIV Infections / drug therapy
  • HIV Infections / virology*
  • Humans
  • Mutation*
  • Polymerase Chain Reaction
  • Sensitivity and Specificity
  • Treatment Failure

Substances

  • Anti-HIV Agents