A cross-sectional study was performed to evaluate the determinants of physical activity in Italy, by analysing data from the Italian Institute of Statistics (ISTAT) study regarding the health status and use of health services of the Italian population in the years 1999-2000. Multiple logistic regression analysis of data highlighted a lower propensity of women, smokers, ex-smokers, and individuals with a lower educational level, to engage in physical activity. These data are useful for planning targeted prevention strategies.