Small-molecule regulation of zebrafish gene expression

Nat Chem Biol. 2007 Mar;3(3):154-5. doi: 10.1038/nchembio858. Epub 2007 Jan 21.

Abstract

The zebrafish has emerged as a versatile model organism for biomedical research, yet its potential has been limited by a lack of conditional reverse-genetic tools. Here we report a chemically inducible gene expression technology that has orthogonality to vertebrate signaling processes, high induction levels, and rapid kinetics. Coupled with tissue-specific promoters, this system provides multidimensional control of gene expression and will enable new models of human disorders and diseases.

Publication types

  • Research Support, N.I.H., Extramural
  • Research Support, Non-U.S. Gov't

MeSH terms

  • Animals
  • Cell Line
  • Embryo, Nonmammalian
  • Gene Expression Regulation / physiology*
  • Genomics / methods*
  • Herpes Simplex Virus Protein Vmw65 / biosynthesis
  • Herpes Simplex Virus Protein Vmw65 / genetics
  • Humans
  • Insecta
  • Receptors, Steroid / genetics
  • Receptors, Steroid / physiology
  • Trans-Activators / genetics
  • Zebrafish / genetics*

Substances

  • Herpes Simplex Virus Protein Vmw65
  • Receptors, Steroid
  • Trans-Activators
  • ecdysone receptor

Associated data

  • PubChem-Substance/17435648
  • PubChem-Substance/17435649
  • PubChem-Substance/17435650
  • PubChem-Substance/17435651
  • PubChem-Substance/17435652
  • PubChem-Substance/17435653
  • PubChem-Substance/17435654