[Immunohistologic investigations of paraffin-embedded lymph nodes after bone marrow transplantation in autopsy samples]

Verh Dtsch Ges Pathol. 1991:75:136-40.
[Article in German]

Abstract

A histological and immunohistochemical analysis of paraffin-embedded lymph nodes from 31 autopsies after bone marrow transplantation (BMT) was performed (20 allogeneic, 10 autologous, 1 syngeneic). Monoclonal antibodies against CD 45RB, CD 20, CD 21, CD 35. CD 43, CD 45RO, CD 76 and Ki-B3, and antisera for detection of S 100-protein and immunoglobulin isotypes were used. None of the lymph nodes showed a regular reconstitution. The lymphoid cells, scattered in a diffuse pattern, were mainly CD 43-positive. Most of them also expressed the CD 45RO antigen. CD 20- and Ki-B3 positive lymphoid cells were nearly absent within the first 100 days after BMT. After that time B cell follicles were detectable in a few cases. Surprisingly, in nearly all cases with infectious complications, numerous plasma cells could be found. The origin of this plasma cells is discussed.

Publication types

  • English Abstract

MeSH terms

  • Antibodies, Monoclonal
  • Antigens, CD / analysis*
  • Autopsy
  • B-Lymphocytes / immunology
  • B-Lymphocytes / pathology
  • Bone Marrow Transplantation / immunology*
  • Bone Marrow Transplantation / pathology
  • Histological Techniques
  • Humans
  • Immune Sera
  • Immunohistochemistry
  • Lymph Nodes / immunology*
  • Lymph Nodes / pathology
  • Paraffin
  • S100 Proteins / analysis
  • T-Lymphocytes / immunology
  • T-Lymphocytes / pathology

Substances

  • Antibodies, Monoclonal
  • Antigens, CD
  • Immune Sera
  • S100 Proteins
  • Paraffin