Isolation and structure elucidation by LC-MS-SPE/NMR: PR toxin- and cuspidatol-related eremophilane sesquiterpenes from Penicillium roqueforti

J Nat Prod. 2007 Jan;70(1):121-3. doi: 10.1021/np060454v.

Abstract

Three eremophilane sesquiterpenes (1, 2, and 3) were isolated from Penicillium roqueforti DAOM 232127, and their structures were established. The new (3S)-3-acetoxyeremophil-1(2),7(11),9(10)-trien-8-one (3) is a likely biosynthetic precursor of PR toxin. 1-Hydroxyeremophil-7(11),9(10)-dien-8-one (1) is related to the immunosuppressant cuspidatol. The application of semihyphenated LC-MS-SPE/NMR to rapidly identify, purify, and elucidate the structures of 1, 2, and 3 is described.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Molecular Structure
  • Naphthols* / chemistry
  • Naphthols* / isolation & purification
  • Naphthols* / pharmacology
  • Nuclear Magnetic Resonance, Biomolecular
  • Penicillium / chemistry*
  • Sesquiterpenes* / chemistry
  • Sesquiterpenes* / isolation & purification
  • Sesquiterpenes* / pharmacology

Substances

  • (3S)-3-acetoxyeremophil-1(2),7(11),9(10)-trien-8-one
  • (3S)-3-acetoxyeremophil-7(11),9(10)-dien-8-one
  • 1-hydroxyeremophil-7(11),9(10)-dien-8-one
  • Naphthols
  • Sesquiterpenes
  • PR Toxin